erbB2 gene silencing and its effect on PTEN in SACC-83 salivary adenoid cystic carcinoma cells.
نویسندگان
چکیده
erbB2 gene plays an important role in carcinoma formation. erbB2 overexpression was observed in many types of tumours, including salivary carcinoma. However, a putative erbB2 and PTEN interaction remains largely unknown in salivary adenoid cystic carcinoma cells. The purpose of this study was to silence erbB2 gene and investigate the functional relationship between erbB2 and PTEN in SACC-83 salivary adenoid cystic carcinoma cells. erbB2-specific siRNAs were transfected into SACC-83 cells using cationic liposome. RT-PCR, immunocytochemistry and Western blotting were employed to detect erbB2 and PTEN expression. Compared with the control groups, erbB2 mRNA expression was decreased in the erbB2-siRNA transfection group, and immunocytochemistry and Western blotting indicated a concordant erbB2 protein reduction. The average optical density values for erbB2 proteins in erbB2-siRNA transfected group were significantly lower than that in the control groups (P<0.05). On the other hand, PTEN expression at both mRNA and protein levels were not significantly affected by erbB2 silencing (P>0.05). In conclusion, the data indicate that siRNA could effectively silence erbB2 gene expression in SACC-83 cells, but PTEN expression appeared unaltered following erbB2 silencing. PTEN expression might not be strictly associated with erbB2 amplification in SACC-83 cells. Future studies will more closely examine the molecular and biological relationships of erbB2 and PTEN in salivary adenoid cystic carcinoma.
منابع مشابه
RNAi knockdown of C-erbB2 expression inhibits salivary gland adenoid cystic carcinoma SACC-83 cell growth in vitro
OBJECTIVE To knockdown the C-erbB2 gene in salivary gland adenoid cystic carcinoma SACC-83 cells using RNA interference, and determine the effect of silencing C-erbB2 on cell proliferation. METHODS C-erbB2-siRNA was transfected into SACC-83 cells. RT-PCR and immunohistochemistry were used to detect C-erbB2 expression in SACC-83 cells. Cell proliferation was measured by the MTT assay and gene ...
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ورودعنوان ژورنال:
- Oncology reports
دوره 24 5 شماره
صفحات -
تاریخ انتشار 2010